Plasmid

Part:BBa_K4687009

Designed by: Yiming Jiang   Group: iGEM23_HBUT-China   (2023-10-02)


pJYS1

The pJYS1 vector is a self-replicating DNA molecule that transfers DNA fragments (target genes) to a recipient cell under a genetically engineered recombinant DNA technology. The pJYS1 vector in this experiment introduced CRISPR components into cells, and editing of target genes was performed by expression of Cas protein and gRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5617
    Illegal EcoRI site found at 5860
    Illegal EcoRI site found at 6736
    Illegal XbaI site found at 4834
    Illegal SpeI site found at 2600
    Illegal SpeI site found at 10554
    Illegal SpeI site found at 10933
    Illegal PstI site found at 3790
    Illegal PstI site found at 5080
    Illegal PstI site found at 5347
    Illegal PstI site found at 6541
    Illegal PstI site found at 8710
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5617
    Illegal EcoRI site found at 5860
    Illegal EcoRI site found at 6736
    Illegal SpeI site found at 2600
    Illegal SpeI site found at 10554
    Illegal SpeI site found at 10933
    Illegal PstI site found at 3790
    Illegal PstI site found at 5080
    Illegal PstI site found at 5347
    Illegal PstI site found at 6541
    Illegal PstI site found at 8710
    Illegal NotI site found at 5947
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5617
    Illegal EcoRI site found at 5860
    Illegal EcoRI site found at 6736
    Illegal BglII site found at 4643
    Illegal BglII site found at 5649
    Illegal BglII site found at 5739
    Illegal BglII site found at 6630
    Illegal BglII site found at 7074
    Illegal BglII site found at 7368
    Illegal BglII site found at 7488
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5617
    Illegal EcoRI site found at 5860
    Illegal EcoRI site found at 6736
    Illegal XbaI site found at 4834
    Illegal SpeI site found at 2600
    Illegal SpeI site found at 10554
    Illegal SpeI site found at 10933
    Illegal PstI site found at 3790
    Illegal PstI site found at 5080
    Illegal PstI site found at 5347
    Illegal PstI site found at 6541
    Illegal PstI site found at 8710
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5617
    Illegal EcoRI site found at 5860
    Illegal EcoRI site found at 6736
    Illegal XbaI site found at 4834
    Illegal SpeI site found at 2600
    Illegal SpeI site found at 10554
    Illegal SpeI site found at 10933
    Illegal PstI site found at 3790
    Illegal PstI site found at 5080
    Illegal PstI site found at 5347
    Illegal PstI site found at 6541
    Illegal PstI site found at 8710
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 9248
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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